pCS2_Firefly_degron-60A
(Plasmid
#200396)
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PurposeA plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200396 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFirefly luciferase - degron
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2_Firefly_degron-60A was a gift from Xiao Wang (Addgene plasmid # 200396 ; http://n2t.net/addgene:200396 ; RRID:Addgene_200396) -
For your References section:
Chemically Modified mocRNAs for Highly Efficient Protein Expression in Mammalian Cells. Aditham A, Shi H, Guo J, Zeng H, Zhou Y, Wade SD, Huang J, Liu J, Wang X. ACS Chem Biol. 2022 Dec 16;17(12):3352-3366. doi: 10.1021/acschembio.1c00569. Epub 2022 Jan 7. 10.1021/acschembio.1c00569 PubMed 34995053