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PX458_3XHA_dFnCas9_KRAB_T2A_eGFP
(Plasmid #200389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX458
  • Backbone size w/o insert (bp) 4243
  • Total vector size (bp) 10320
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PX458_3XHA_dFnCas9_KRAB_eGFP
  • Alt name
    Dead FnCas9 with 3XHA tag, KRAB domain and eGFP for mammalian expression
  • Alt name
    dFnCas9b
  • Species
    Synthetic; Francisella novicida U112
  • Insert Size (bp)
    6077
  • GenBank ID
    NZ_CP009607.1
  • Promoter CBH
  • Tags / Fusion Proteins
    • 3xHA-NLS (N terminal on insert)
    • SV40NLS-KRAB-T2A-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Eco53kI (not destroyed)
  • 5′ sequencing primer CTGGCTTATAGGTCTAGGCGCGTGAAGATCAAAAG
  • 3′ sequencing primer CTTTTGATCTTCACGCGCCTAGACCTATAAGCCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Subcloned from PX408, a gift from Feng Zhang's Lab

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PX458_3XHA_dFnCas9_KRAB_T2A_eGFP was a gift from Debojyoti Chakraborty (Addgene plasmid # 200389 ; http://n2t.net/addgene:200389 ; RRID:Addgene_200389)
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