pHiUGE smFP-V5 donor (all-in-one) ORF0
(Plasmid
#200386)
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PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHiUGE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namespaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
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SpeciesOther
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MutationNA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SapI (not destroyed)
- 3′ cloning site SapI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.10.06.511211v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHiUGE smFP-V5 donor (all-in-one) ORF0 was a gift from Scott Soderling (Addgene plasmid # 200386 ; http://n2t.net/addgene:200386 ; RRID:Addgene_200386) -
For your References section:
Proximity analysis of native proteomes reveals phenotypic modifiers in a mouse model of autism and related neurodevelopmental conditions. Gao Y, Shonai D, Trn M, Zhao J, Soderblom EJ, Garcia-Moreno SA, Gersbach CA, Wetsel WC, Dawson G, Velmeshev D, Jiang YH, Sloofman LG, Buxbaum JD, Soderling SH. Nat Commun. 2024 Aug 9;15(1):6801. doi: 10.1038/s41467-024-51037-x. 10.1038/s41467-024-51037-x PubMed 39122707