pHiUGE smFP-V5 donor ORF2
(Plasmid
#200382)
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PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200382 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHiUGE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namespaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
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SpeciesOther
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MutationNA
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHiUGE smFP-V5 donor ORF2 was a gift from Scott Soderling (Addgene plasmid # 200382 ; http://n2t.net/addgene:200382 ; RRID:Addgene_200382) -
For your References section:
Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering. Gao Y, Hisey E, Bradshaw TWA, Erata E, Brown WE, Courtland JL, Uezu A, Xiang Y, Diao Y, Soderling SH. Neuron. 2019 Aug 21;103(4):583-597.e8. doi: 10.1016/j.neuron.2019.05.047. Epub 2019 Jul 1. 10.1016/j.neuron.2019.05.047 PubMed 31272828