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Addgene

pHiUGE GS-gRNA (hSyn-Cre) mTubb3
(Plasmid #200369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200369 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHiUGE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mouse Tubb3 gRNA and Cre
  • gRNA/shRNA sequence
    Unspecified
  • Species
    M. musculus (mouse)
  • Mutation
    NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SapI (destroyed during cloning)
  • 3′ cloning site SapI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHiUGE GS-gRNA (hSyn-Cre) mTubb3 was a gift from Scott Soderling (Addgene plasmid # 200369 ; http://n2t.net/addgene:200369 ; RRID:Addgene_200369)

  • For your References section:

    Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering. Gao Y, Hisey E, Bradshaw TWA, Erata E, Brown WE, Courtland JL, Uezu A, Xiang Y, Diao Y, Soderling SH. Neuron. 2019 Aug 21;103(4):583-597.e8. doi: 10.1016/j.neuron.2019.05.047. Epub 2019 Jul 1. 10.1016/j.neuron.2019.05.047 PubMed 31272828
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