pCFJ108 - pENTR221 - [cbr-unc-119(+)]
(Plasmid
#200367)
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Purposecbr-unc-119 rescue gene for use as co-injection marker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200367 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name[cbr-unc-119(+)]
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cbr-unc-119 rescue marker. Can be used as co-injection marker. Rescues C. elegans unc-119 mutants.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFJ108 - pENTR221 - [cbr-unc-119(+)] was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 200367 ; http://n2t.net/addgene:200367 ; RRID:Addgene_200367) -
For your References section:
Characterizing a standardized BioPart for PVQ-specific expression in C. elegans. AlHarbi S, Frokjaer-Jensen C. MicroPubl Biol. 2023 Jun 22;2023:10.17912/micropub.biology.000870. doi: 10.17912/micropub.biology.000870. eCollection 2023. 10.17912/micropub.biology.000870 PubMed 37426742