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Addgene

pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet]
(Plasmid #200365)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Twist Amp High
  • Backbone manufacturer
    Twist Biosciences
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MOdular Safe harbor Transgene Insertion (mosTI) (El Mouridi et al., 2022). Standard cloning vector for unc-119, wrmScarlet based mosTI insertions with MCS and Golden Gate cloning sites. Screen for unc-119 rescue and wrmScarlet after single-copy insertion. For more information visit www.wormbuilder.org.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet] was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 200365 ; http://n2t.net/addgene:200365 ; RRID:Addgene_200365)
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