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Addgene

H6GB1tev-IntC-P53(304-393)
(Plasmid #200315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p53 (304-393)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    586
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter T7
  • Tags / Fusion Proteins
    • His6GB1tev (N terminal on insert)
    • designed intein C (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The intein sequence was taken from Addgene #20177

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H6GB1tev-IntC-P53(304-393) was a gift from Peter Wright (Addgene plasmid # 200315 ; http://n2t.net/addgene:200315 ; RRID:Addgene_200315)

  • For your References section:

    Mapping Interactions of the Intrinsically Disordered C-Terminal Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR. Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE. Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 10.1021/acs.biochem.2c00528. Epub 2022 Nov 15. 10.1021/acs.biochem.2c00528 PubMed 36380579
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