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Addgene

OaAEP1_C247A
(Plasmid #200307)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200307 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHYRSF53
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Asparaginyl endopeptidase from Oldenlandia affinis
  • Species
    Oldenlandia affinis
  • Mutation
    Cysteine 247 to Alanine
  • Promoter T7
  • Tag / Fusion Protein
    • Smt3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OaAEP1_C247A was a gift from Artur Krężel (Addgene plasmid # 200307 ; http://n2t.net/addgene:200307 ; RRID:Addgene_200307)

  • For your References section:

    OaAEP1 Ligase-Assisted Chemoenzymatic Synthesis of Full Cysteine-Rich Metal-Binding Cyanobacterial Metallothionein SmtA. Antonenko A, Singh AK, Mosna K, Krezel A. Bioconjug Chem. 2023 Mar 15;34(4):719-27. doi: 10.1021/acs.bioconjchem.3c00037. 10.1021/acs.bioconjchem.3c00037 PubMed 36921066
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