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Addgene

MT2A_beta-Villin
(Plasmid #200301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200301 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTyb21
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-domain of metallothionein 2a in fusion with HP-35
  • Species
    H. sapiens (human)
  • Entrez Gene
    MT2A (a.k.a. MT-2, MT-II, MT2)
  • Promoter T7
  • Tag / Fusion Protein
    • Chiting Binding Domanin-Intein (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SapI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MT2A_beta-Villin was a gift from Artur Krężel (Addgene plasmid # 200301 ; http://n2t.net/addgene:200301 ; RRID:Addgene_200301)

  • For your References section:

    The connection of alpha- and beta-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties. Singh AK, Pomorski A, Wu S, Peris-Diaz MD, Czepczynska-Krezel H, Krezel A. Metallomics. 2023 Jun 1;15(6):mfad029. doi: 10.1093/mtomcs/mfad029. 10.1093/mtomcs/mfad029 PubMed 37147085
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