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Addgene

SpMTA Purple Sea Urchin Metallothionein
(Plasmid #200292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTYB21
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gene of metallothionein with Chiting Binding Domanin-Intein
  • Species
    Strongylocentrotus purpuratus
  • GenBank ID
    XP_030846082.1
  • Tag / Fusion Protein
    • Chiting Binding Domanin-Intein (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpMTA Purple Sea Urchin Metallothionein was a gift from Artur Krężel (Addgene plasmid # 200292 ; http://n2t.net/addgene:200292 ; RRID:Addgene_200292)

  • For your References section:

    Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn. Mosna K, Jurczak K, Krezel A. Metallomics. 2023 Oct 4;15(10):mfad061. doi: 10.1093/mtomcs/mfad061. 10.1093/mtomcs/mfad061 PubMed 37804185
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