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Addgene

Pum NaV1.4-N432T
(Plasmid #200284)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200284 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pitohui uropygialis meridionalis NaV1.4
  • Species
    Pitohui uropygialis meridionalis
  • Mutation
    N432T
  • GenBank ID
    MZ545383.1
  • Promoter CMV and T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AflII (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer bgh reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pum NaV1.4-N432T was a gift from Dan Minor (Addgene plasmid # 200284 ; http://n2t.net/addgene:200284 ; RRID:Addgene_200284)

  • For your References section:

    Evidence that toxin resistance in poison birds and frogs is not rooted in sodium channel mutations and may rely on "toxin sponge" proteins. Abderemane-Ali F, Rossen ND, Kobiela ME, Craig RA, Garrison CE, Chen Z, Colleran CM, O'Connell LA, Du Bois J, Dumbacher JP, Minor DL. J Gen Physiol. 2021 Sep 6;153(9):e202112872. doi: 10.1085/jgp.202112872. Epub 2021 Aug 5. 10.1085/jgp.202112872 PubMed 34351379
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