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Addgene

pET30-10His-FKBP(f36v)-SNAP
(Plasmid #200282)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200282 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositor recommends using Rosetta for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FKBP-SNAPtag
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-10His-FKBP(f36v)-SNAP was a gift from Kristina Ganzinger (Addgene plasmid # 200282 ; http://n2t.net/addgene:200282 ; RRID:Addgene_200282)

  • For your References section:

    Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions. Niederauer C, Nguyen C, Wang-Henderson M, Stein J, Strauss S, Cumberworth A, Stehr F, Jungmann R, Schwille P, Ganzinger KA. Nat Commun. 2023 Jul 19;14(1):4345. doi: 10.1038/s41467-023-40065-8. 10.1038/s41467-023-40065-8 PubMed 37468504
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