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pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
(Plasmid #200277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbE8K
  • Backbone size w/o insert (bp) 3487
  • Total vector size (bp) 7504
  • Modifications to backbone
    Elements for arabinose-inducible expression were replaced with the alanine racemase gene and kanamycin gene flanked with FRT sites
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CadC
  • Species
    Escherichia coli
  • Insert Size (bp)
    1539

Gene/Insert 2

  • Gene/Insert name
    NanR
  • Species
    Escherichia coli
  • Insert Size (bp)
    792

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP was a gift from Matthew Chang (Addgene plasmid # 200277 ; http://n2t.net/addgene:200277 ; RRID:Addgene_200277)

  • For your References section:

    Engineering probiotics to inhibit Clostridioides difficile infection by dynamic regulation of intestinal metabolism. Koh E, Hwang IY, Lee HL, De Sotto R, Lee JWJ, Lee YS, March JC, Chang MW. Nat Commun. 2022 Jul 4;13(1):3834. doi: 10.1038/s41467-022-31334-z. 10.1038/s41467-022-31334-z PubMed 35787625
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