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Addgene

pcDNA3.1-Vinculin_TurboID_Venus
(Plasmid #200272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5379
  • Total vector size (bp) 10280
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vinculin_TurboID_Venus
  • Alt name
    Vin-TurboID-Ven
  • Species
    G. gallus (chicken), Synthetic
  • Insert Size (bp)
    4900
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTAAACGGCCACAAGTTCAGC
  • 3′ sequencing primer GACGTAGCCTTCGGGCAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Vinculin_TurboID_Venus was a gift from Brenton Hoffman (Addgene plasmid # 200272 ; http://n2t.net/addgene:200272 ; RRID:Addgene_200272)

  • For your References section:

    Identifying constitutive and context-specific molecular-tension-sensitive protein recruitment within focal adhesions. Tao A, LaCroix AS, Shoyer TC, Venkatraman V, Xu KL, Feiger B, Hoffman BD. Dev Cell. 2023 Mar 10:S1534-5807(23)00074-6. doi: 10.1016/j.devcel.2023.02.015. 10.1016/j.devcel.2023.02.015 PubMed 36924770
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