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pBI-eGFP-Rab31
(Plasmid #200265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBI
  • Backbone manufacturer
    Clontech/Takara Bio
  • Backbone size w/o insert (bp) 4358
  • Total vector size (bp) 5663
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab31 (human cDNA)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    588
  • Entrez Gene
    RAB31 (a.k.a. Rab22B)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer atgatggcgatacgggag
  • 3′ sequencing primer tcaacagcaccggcg
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rab31 cDNA was from UMR cDNA resource center

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI-eGFP-Rab31 was a gift from Guangpu Li (Addgene plasmid # 200265 ; http://n2t.net/addgene:200265 ; RRID:Addgene_200265)

  • For your References section:

    A novel membrane targeting domain mediates the endosomal or Golgi localization specificity of small GTPases Rab22 and Rab31. Banworth MJ, Liang Z, Li G. J Biol Chem. 2022 Sep;298(9):102281. doi: 10.1016/j.jbc.2022.102281. Epub 2022 Jul 19. 10.1016/j.jbc.2022.102281 PubMed 35863437
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