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Addgene

pXY349
(Plasmid #200262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIQ
  • Backbone size w/o insert (bp) 4674
  • Total vector size (bp) 6693
  • Modifications to backbone
    modify the −35 region of the lacIQ promoter from GTGCAA to TTGACA.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FtsW-tagRFP
  • Insert Size (bp)
    2019
  • Entrez Gene
    ftsW (a.k.a. b0089, ECK0090)
  • Promoter LacI
  • Tag / Fusion Protein
    • tagRFP (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ctttcgtcttcacctcgagaaatc
  • 3′ sequencing primer gctaattaagcttggctgcaggt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXY349 was a gift from Jie Xiao (Addgene plasmid # 200262 ; http://n2t.net/addgene:200262 ; RRID:Addgene_200262)

  • For your References section:

    A two-track model for the spatiotemporal coordination of bacterial septal cell wall synthesis revealed by single-molecule imaging of FtsW. Yang X, McQuillen R, Lyu Z, Phillips-Mason P, De La Cruz A, McCausland JW, Liang H, DeMeester KE, Santiago CC, Grimes CL, de Boer P, Xiao J. Nat Microbiol. 2021 May;6(5):584-593. doi: 10.1038/s41564-020-00853-0. Epub 2021 Jan 25. 10.1038/s41564-020-00853-0 PubMed 33495624
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