pENTR4-myc-nuc (w556-1)
(Plasmid #20026)

• Purpose: (Empty Backbone) Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: pENTR4
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 2449
• Vector type: Mammalian Expression ; Gateway Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: no insert
• Tags / Fusion Proteins:
  • NLS (C terminal on backbone)
  • Myc (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: Nco I (not destroyed)
• 3′ cloning site: Bam HI (destroyed during cloning)
• 5′ sequencing primer: ENTRforw
• 3′ sequencing primer: ENTRrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Nuc-myc derived from pCMV/myc/nuc (Invitrogen)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry