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Addgene

2333_Cas9-CtIP-dnRNF168
(Plasmid #200254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPIX
  • Backbone size w/o insert (bp) 3197
  • Total vector size (bp) 7214
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-CtIP-dnRNF168
  • Species
    H. sapiens (human); Streptococcus pyogenes
  • Insert Size (bp)
    8661
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATCCACTTTGCCTTTCTCTCCACAGG
  • 3′ sequencing primer cctcgactgtgccttcta
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2333_Cas9-CtIP-dnRNF168 was a gift from Claudio Mussolino (Addgene plasmid # 200254 ; http://n2t.net/addgene:200254 ; RRID:Addgene_200254)

  • For your References section:

    A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells. Carusillo A, Haider S, Schafer R, Rhiel M, Turk D, Chmielewski KO, Klermund J, Mosti L, Andrieux G, Schafer R, Cornu TI, Cathomen T, Mussolino C. Nucleic Acids Res. 2023 May 22;51(9):4660-4673. doi: 10.1093/nar/gkad255. 10.1093/nar/gkad255 PubMed 37070192
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