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Addgene

Pum NaVB2
(Plasmid #200231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES-eGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pitohui uropygialis meridionalis NaV beta 2
  • Species
    Pitohui uropygialis meridionalis
  • GenBank ID
    MZ545385.1
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer bgh reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pum NaVB2 was a gift from Dan Minor (Addgene plasmid # 200231 ; http://n2t.net/addgene:200231 ; RRID:Addgene_200231)

  • For your References section:

    Evidence that toxin resistance in poison birds and frogs is not rooted in sodium channel mutations and may rely on "toxin sponge" proteins. Abderemane-Ali F, Rossen ND, Kobiela ME, Craig RA, Garrison CE, Chen Z, Colleran CM, O'Connell LA, Du Bois J, Dumbacher JP, Minor DL. J Gen Physiol. 2021 Sep 6;153(9):e202112872. doi: 10.1085/jgp.202112872. Epub 2021 Aug 5. 10.1085/jgp.202112872 PubMed 34351379
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