pNeoR2
(Plasmid
#200223)
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Purpose(Empty Backbone) NeoR nls-scarlet marked rapid RMCE integration vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHygR2
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Modifications to backboneNeoR replaces HygR
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Vector typeWorm Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNeoR2 was a gift from Michael Nonet (Addgene plasmid # 200223 ; http://n2t.net/addgene:200223 ; RRID:Addgene_200223) -
For your References section:
Rapid generation of C. elegans single-copy transgenes combining RMCE and drug selection. Nonet ML. Genetics. 2023 Apr 20:iyad072. doi: 10.1093/genetics/iyad072. 10.1093/genetics/iyad072 PubMed 37079426