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Addgene

pRAW-459
(Plasmid #200215)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    2S
  • Backbone size w/o insert (bp) 2819
  • Total vector size (bp) 7257
  • Modifications to backbone
    site-directed mutagenesis of Plasmid #89240
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PaCas1, PaCas2-3
  • Alt name
    Cas1, Cas2/3, Cas3
  • Species
    Pseudomonas aeruginosa UCBPP-PA14
  • Insert Size (bp)
    4438
  • Mutation
    Cas1 K214A mutation
  • GenBank ID
    WP_003139228 WP_003139227
  • Tag / Fusion Protein
    • Strep II-TEV on Cas1 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRAW-459 was a gift from Blake Wiedenheft (Addgene plasmid # 200215 ; http://n2t.net/addgene:200215 ; RRID:Addgene_200215)

  • For your References section:

    Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays. Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B. Nat Struct Mol Biol. 2023 Nov;30(11):1675-1685. doi: 10.1038/s41594-023-01097-2. Epub 2023 Sep 14. 10.1038/s41594-023-01097-2 PubMed 37710013
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