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Addgene

HSF1 KO
(Plasmid #200207)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200207 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSF1 KO
  • gRNA/shRNA sequence
    TCTCGGACGAAAGCAGGCAG, ATGTCCTAGGGATAGGTTGG
  • Species
    M. musculus (mouse)
  • Mutation
    None
  • Entrez Gene
    Hsf1 (a.k.a. HSTF, HSTF 1, Hsf1alpha, Hsf1beta)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HSF1 KO was a gift from Sheila Teves (Addgene plasmid # 200207 ; http://n2t.net/addgene:200207 ; RRID:Addgene_200207)

  • For your References section:

    Heat shock transcription factors demonstrate a distinct mode of interaction with mitotic chromosomes. Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS. Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304. 10.1093/nar/gkad304 PubMed 37114996
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