Skip to main content
Addgene

HA-Halo-H2DBD
(Plasmid #200206)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200206 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    HA-3X-Flag-Halo-TEV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HA-Halo-H2DBD
  • Species
    M. musculus (mouse)
  • Mutation
    HSF2 (1-366; 15 bp linker; 972-1017)
  • Entrez Gene
    Hsf2
  • Promoter EF_1alpha
  • Tag / Fusion Protein
    • Halo; HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.10.05.511012v1 for bioRxiv preprint.

Addgene's NGS results found a discrepancy that removed NheI and BmtI restriction sites, but plasmid function is not affected.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-Halo-H2DBD was a gift from Sheila Teves (Addgene plasmid # 200206 ; http://n2t.net/addgene:200206 ; RRID:Addgene_200206)

  • For your References section:

    Heat shock transcription factors demonstrate a distinct mode of interaction with mitotic chromosomes. Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS. Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304. 10.1093/nar/gkad304 PubMed 37114996
Related items: