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Addgene

H1DBD-HR-Halo
(Plasmid #200204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TEV-Halo-1X-Flag
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H1DBD-HR-Halo
  • Species
    M. musculus (mouse)
  • Mutation
    HSF1 (1-672)
  • Entrez Gene
    Hsf1 (a.k.a. HSTF, HSTF 1, Hsf1alpha, Hsf1beta)
  • Promoter EF_1alpha
  • Tag / Fusion Protein
    • Halo (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H1DBD-HR-Halo was a gift from Sheila Teves (Addgene plasmid # 200204 ; http://n2t.net/addgene:200204 ; RRID:Addgene_200204)

  • For your References section:

    Heat shock transcription factors demonstrate a distinct mode of interaction with mitotic chromosomes. Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS. Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304. 10.1093/nar/gkad304 PubMed 37114996
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