Halo-Sox13
(Plasmid
#200194)
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PurposeExpression of full length Sox13
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 200194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbone3X-Flag-Halo-TEV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHalo-Sox13
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SpeciesM. musculus (mouse)
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MutationNone
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Entrez GeneSox13 (a.k.a. Sox-13)
- Promoter EF_1alpha
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Tag
/ Fusion Protein
- Halo (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.10.05.511012v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Halo-Sox13 was a gift from Sheila Teves (Addgene plasmid # 200194 ; http://n2t.net/addgene:200194 ; RRID:Addgene_200194) -
For your References section:
Heat shock transcription factors demonstrate a distinct mode of interaction with mitotic chromosomes. Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS. Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304. 10.1093/nar/gkad304 PubMed 37114996