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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonecFlag pcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5-alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNfkb2
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Alt namep52
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SpeciesM. musculus (mouse)
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Insert Size (bp)1215
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MutationTruncated at the p100 ligation site. It codes for Nfkb2 residues 1-405.
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GenBank IDNP_062281
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Entrez GeneNfkb2 (a.k.a. NF-kappaB2, lyt, p49, p49/p100, p50B, p52)
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Tag
/ Fusion Protein
- Flag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p52 cFlag pcDNA3 was a gift from Stephen Smale (Addgene plasmid # 20019 ; http://n2t.net/addgene:20019 ; RRID:Addgene_20019)