p50 cFlag pcDNA3
(Plasmid #20018)

• Depositing Lab: Stephen Smale
• Publication: NFkappaB EMSA Studies (unpublished)

Backbone

• Vector backbone: cFlag pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5-alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nfkb1
• Alt name: p50
• Species: M. musculus (mouse)
• Insert Size (bp): 1284
• Mutation: Truncated at the p105 ligation site. It codes for Nfkb1 residues 1-428.
• Entrez Gene: Nfkb1 (a.k.a. NF-KB1, NF-kappaB, NF-kappaB1, p105, p50, p50/p105)
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).