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Addgene

pCast
(Plasmid #200169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200169 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    not applicable
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    Nourseothricin (clonNAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCast was a gift from Yong-Su Jin (Addgene plasmid # 200169 ; http://n2t.net/addgene:200169 ; RRID:Addgene_200169)

  • For your References section:

    Domesticating a food spoilage yeast into an organic acid-tolerant metabolic engineering host: Lactic acid production by engineered Zygosaccharomyces bailii. Kuanyshev N, Rao CV, Dien B, Jin YS. Biotechnol Bioeng. 2021 Jan;118(1):372-382. doi: 10.1002/bit.27576. Epub 2020 Oct 8. 10.1002/bit.27576 PubMed 33030791
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