pcS-E626-C1C2
(Plasmid #200162)

• Purpose: This plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.
• Depositing Lab: Masako Harada
• Publication: Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2). pii: bioengineering9020056. doi: 10.3390/bioengineering9020056.

Backbone

• Vector backbone: pcS-p88-C1C2
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: E626
• Alt name: EGFR-targeting monobody
• Species: H. sapiens (human)
• Insert Size (bp): 286
• Mutation: sequence adopted from doi: 10.1371/journal.pone.0138956
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: C1C2 domain of lactadherin
• Alt name: hC1C2
• Species: H. sapiens (human)
• Insert Size (bp): 963
• GenBank ID: NC_000015.10
• Tag / Fusion Protein:
  • HIS (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry