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Addgene

pET-APOBEC1-YTH
(Plasmid #200158)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 MBP TEV LIC cloning vector
  • Backbone manufacturer
    Scott Gradia
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 7800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APOBEC1-YTH
  • Species
    H. sapiens (human), R. norvegicus (rat)
  • Mutation
    Codon optimized for E coli expression
  • Promoter LacZ
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • 6xHis (N terminal on insert)
    • Maltose Binding Protein (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-APOBEC1-YTH was a gift from Kate Meyer (Addgene plasmid # 200158 ; http://n2t.net/addgene:200158 ; RRID:Addgene_200158)

  • For your References section:

    Improved Methods for Deamination-Based m(6)A Detection. Zhu H, Yin X, Holley CL, Meyer KD. Front Cell Dev Biol. 2022 Apr 27;10:888279. doi: 10.3389/fcell.2022.888279. eCollection 2022. 10.3389/fcell.2022.888279 PubMed 35573664
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