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Addgene

pHHYTK122
(Plasmid #200146)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Backbone manufacturer
    John Dueber Lab, https://doi.org/10.1021/sb500366v
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP1-10
  • Species
    A. victoria
  • Insert Size (bp)
    639

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHHYTK122 was a gift from Johannes Herrmann (Addgene plasmid # 200146 ; http://n2t.net/addgene:200146 ; RRID:Addgene_200146)

  • For your References section:

    A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeast Saccharomyces cerevisiae. Simakin P, Koch C, Herrmann JM. Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. eCollection 2023 Apr 3. 10.15698/mic2023.04.794 PubMed 37009624
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