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pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-Fluc
(Plasmid #200128)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCxIP
  • Backbone size w/o insert (bp) 7138
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flip-nluc(LgBiT1-8)CP[CoVA(SA)] P2A firefly luciferase
  • Species
    Synthetic
  • Insert Size (bp)
    2844
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-Fluc was a gift from Eiji Morita (Addgene plasmid # 200128 ; http://n2t.net/addgene:200128 ; RRID:Addgene_200128)

  • For your References section:

    A highly sensitive NanoLuc-based protease biosensor for detecting apoptosis and SARS-CoV-2 infection. Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E. Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4. 10.1038/s41598-023-28984-4 PubMed 36720982
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