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Addgene

pCAG.Myc-SARS2_3CL(C145A)
(Plasmid #200121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-Myc
  • Backbone size w/o insert (bp) 4872
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 3CL protease
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    926
  • Mutation
    C145A
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter CAG
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG.Myc-SARS2_3CL(C145A) was a gift from Eiji Morita (Addgene plasmid # 200121 ; http://n2t.net/addgene:200121 ; RRID:Addgene_200121)

  • For your References section:

    A highly sensitive NanoLuc-based protease biosensor for detecting apoptosis and SARS-CoV-2 infection. Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E. Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4. 10.1038/s41598-023-28984-4 PubMed 36720982
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