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Addgene

RelA cFlag pcDNA3
(Plasmid #20012)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20012 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    cFlag pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5-alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RelA
  • Alt name
    p65
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1650
  • GenBank ID
    NM_009045
  • Entrez Gene
    Rela (a.k.a. p65, p65 NF-kappa B, p65 NFkB)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See cFlag pcDNA plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RelA cFlag pcDNA3 was a gift from Stephen Smale (Addgene plasmid # 20012 ; http://n2t.net/addgene:20012 ; RRID:Addgene_20012)

  • For your References section:

    A c-Rel subdomain responsible for enhanced DNA-binding affinity and selective gene activation. Sanjabi S, Williams KJ, Saccani S, Zhou L, Hoffmann A, Ghosh G, Gerondakis S, Natoli G, Smale ST. Genes Dev. 2005 Sep 15. 19(18):2138-51. 10.1101/gad.1329805 PubMed 16166378
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