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Addgene

pKrox24(MapErk)Luc
(Plasmid #200112)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.26
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MapErk sequence in minimal promoter
  • Species
    Synthetic
  • Mutation
    highly modified sequence
  • Promoter 5 copies of designed MapErk sequence in minimal promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKrox24(MapErk)Luc was a gift from Pavel Krejčí (Addgene plasmid # 200112 ; http://n2t.net/addgene:200112 ; RRID:Addgene_200112)

  • For your References section:

    One reporter for in-cell activity profiling of majority of protein kinase oncogenes. Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova Bosakova M, Trantirek L, Mayer J, Krejci P. Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536. 10.7554/eLife.21536 PubMed 28199182
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