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Addgene

pKrox24(2xD-E_inD)Luc
(Plasmid #200109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200109 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.17
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    modified EGR1 promoter
  • Species
    Synthetic
  • Mutation
    highly modified sequence
  • Promoter 2 copies of D-E element in front of EGR1 promoter fragment D, no other promoter elements

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please Note: Plasmid contains an A to G nucleotide mutation in one of the three D elements of the EGR1D promoter and a three nucleotide insertion between the promoter and luciferase. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKrox24(2xD-E_inD)Luc was a gift from Pavel Krejčí (Addgene plasmid # 200109 ; http://n2t.net/addgene:200109 ; RRID:Addgene_200109)

  • For your References section:

    One reporter for in-cell activity profiling of majority of protein kinase oncogenes. Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova Bosakova M, Trantirek L, Mayer J, Krejci P. Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536. 10.7554/eLife.21536 PubMed 28199182
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