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Addgene

E228Q Vps4A-HA
(Plasmid #200087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pINDUCER20
  • Backbone size w/o insert (bp) 11794
  • Total vector size (bp) 11611
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS4A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3701
  • Mutation
    E228Q
  • GenBank ID
    NM_013245.3
  • Entrez Gene
    VPS4A (a.k.a. CIMDAG, SKD1, SKD1A, SKD2, VPS4, VPS4-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E228Q Vps4A-HA was a gift from Jason MacGurn (Addgene plasmid # 200087 ; http://n2t.net/addgene:200087 ; RRID:Addgene_200087)

  • For your References section:

    Lysosomal trafficking of the glucose transporter GLUT1 requires sequential regulation by TXNIP and ubiquitin. Qualls-Histed SJ, Nielsen CP, MacGurn JA. iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. eCollection 2023 Mar 17. 10.1016/j.isci.2023.106150 PubMed 36890792
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