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PGK EGFP-PPM1H-WT
(Plasmid #200084)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200084 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFPc1/PGK
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPM1H
  • Alt name
    ARHCL1, NERPP-2C, URCC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1559
  • GenBank ID
    NM_020700.1
  • Entrez Gene
    PPM1H (a.k.a. ARHCL1, NERPP-2C, URCC2)
  • Promoter PGK
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (unknown if destroyed)
  • 3′ cloning site SmaI, PmeI (unknown if destroyed)
  • 5′ sequencing primer 5'-AGATGAACTTCAGGGTCAGC
  • 3′ sequencing primer 5'-CTTTATTTGTAACCATTATAAGCTGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MRC PPU #DU62939

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGK EGFP-PPM1H-WT was a gift from Erika Holzbaur (Addgene plasmid # 200084 ; http://n2t.net/addgene:200084 ; RRID:Addgene_200084)

  • For your References section:

    Regulatory imbalance between LRRK2 kinase, PPM1H phosphatase, and ARF6 GTPase disrupts the axonal transport of autophagosomes. Dou D, Smith EM, Evans CS, Boecker CA, Holzbaur ELF. Cell Rep. 2023 May 1;42(5):112448. doi: 10.1016/j.celrep.2023.112448. 10.1016/j.celrep.2023.112448 PubMed 37133994
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