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pmKO-imp α
(Plasmid #200082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmKO1-MC1
  • Backbone manufacturer
    MBL
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 5700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    importin α
  • Alt name
    KPNA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    KPNA2 (a.k.a. IPOA1, PTAC58, QIP2, RCH1, SRP1-alpha, SRP1alpha)
  • Promoter CMV
  • Tag / Fusion Protein
    • mKO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer mKO C-terminal side
  • 3′ sequencing primer SV40 poly A side
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The DNA fragment of imp α is the same as the one cloned in pEGFP-imp α
Plasmid vector pmKO1-MC1 is the same as the pmKO-AF8 except for the cloning sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmKO-imp α was a gift from Kenji Sugimoto (Addgene plasmid # 200082 ; http://n2t.net/addgene:200082 ; RRID:Addgene_200082)

  • For your References section:

    A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin alpha as fusion tag. Nakagawa C, Nishimura S, Senda-Murata K, Sugimoto K. Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 10.1271/bbb.110677. Epub 2012 Feb 7. 10.1271/bbb.110677 PubMed 22313767
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