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Addgene

pHygO2
(Plasmid #200077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHygG2
  • Backbone manufacturer
    Stratagene Cloning Systems
  • Modifications to backbone
    removed nls-mNG and myo-2 '3 UTR.
  • Vector type
    Worm Expression ; Recombination Mediated Cassette Exchange
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHygO2 was a gift from Michael Nonet (Addgene plasmid # 200077 ; http://n2t.net/addgene:200077 ; RRID:Addgene_200077)

  • For your References section:

    Rapid generation of C. elegans single-copy transgenes combining RMCE and drug selection. Nonet ML. Genetics. 2023 Apr 20:iyad072. doi: 10.1093/genetics/iyad072. 10.1093/genetics/iyad072 PubMed 37079426
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