pHygCG2r
(Plasmid
#200074)
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Purpose(Empty Backbone) hygR selection mNG marked rapid RMCE integration vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHygCG2
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Modifications to backboneSapI Golden Gate cloning site is opposite orientation to pHygCG2.
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Vector typeWorm Expression ; Recombination Mediated Cassette Exchange
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHygCG2r was a gift from Michael Nonet (Addgene plasmid # 200074 ; http://n2t.net/addgene:200074 ; RRID:Addgene_200074) -
For your References section:
Rapid generation of C. elegans single-copy transgenes combining RMCE and drug selection. Nonet ML. Genetics. 2023 Apr 20:iyad072. doi: 10.1093/genetics/iyad072. 10.1093/genetics/iyad072 PubMed 37079426