pHygR2
(Plasmid
#200071)
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Purpose(Empty Backbone) hygR selection nls-mScarlet marked rapid RMCE integration vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHygG2
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Modifications to backbonenls-mNG replaced with nls-mScarlet.
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Vector typeWorm Expression ; Recombination Mediated Cassette Exchange
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHygR2 was a gift from Michael Nonet (Addgene plasmid # 200071 ; http://n2t.net/addgene:200071 ; RRID:Addgene_200071) -
For your References section:
Rapid generation of C. elegans single-copy transgenes combining RMCE and drug selection. Nonet ML. Genetics. 2023 Apr 20:iyad072. doi: 10.1093/genetics/iyad072. 10.1093/genetics/iyad072 PubMed 37079426