piggyBAC-U6-GFP-WPRE
(Plasmid
#200030)
-
Purpose(Empty Backbone) piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepiggyBAC-5xTAPE-1-BlastR
- Backbone size (bp) 6000
-
Modifications to backboneGene inserts with U6 promoter and GFP (EF1a promoter, WPRE, and SV40-polyA element).
-
Vector typeMammalian Expression
- Promoter U6, EF1a
-
Tag
/ Fusion Protein
- GFP
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
piggyBAC-U6-GFP-WPRE was a gift from Jay Shendure (Addgene plasmid # 200030 ; http://n2t.net/addgene:200030 ; RRID:Addgene_200030)
