LentiCROP-PuroR-P2A-GFP-U6
(Plasmid
#200029)
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Purpose(Empty Backbone) Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCROPseq-Guide-Puro
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Backbone manufacturerChristoph Bock
- Backbone size (bp) 10000
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Modifications to backboneChanged PuroR to PuroR-P2A-GFP and removed sgRNA scaffold to allow pegRNA cloning.
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- PuroR-P2A-GFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LentiCROP-PuroR-P2A-GFP-U6 was a gift from Jay Shendure (Addgene plasmid # 200029 ; http://n2t.net/addgene:200029 ; RRID:Addgene_200029)
