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Addgene

pLVX-TetOne-zeo_3xHA-TurboID-NLS
(Plasmid #200022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 8997
  • Total vector size (bp) 10140
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xHA-TurboID-NLS
  • Insert Size (bp)
    1143
  • Promoter TRE3Gs

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tcccgtatacaccggatgtacccgtatgatg
  • 3′ sequencing primer gaggtggtctggatctcacaccttcctcttcttcttggggtcc
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.01.29.526158 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOne-zeo_3xHA-TurboID-NLS was a gift from Britt Glaunsinger (Addgene plasmid # 200022 ; http://n2t.net/addgene:200022 ; RRID:Addgene_200022)

  • For your References section:

    The viral packaging motor potentiates Kaposi's sarcoma-associated herpesvirus gene expression late in infection. McCollum CO, Didychuk AL, Liu D, Murray-Nerger LA, Cristea IM, Glaunsinger BA. PLoS Pathog. 2023 Apr 17;19(4):e1011163. doi: 10.1371/journal.ppat.1011163. eCollection 2023 Apr. 10.1371/journal.ppat.1011163 PubMed 37068108
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