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pCK083 desmaMCS:minpromEGFP,exorh:mCherry
(Plasmid #200016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2pA2
  • Vector type
    zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v2 for bioRxiv preprint.

Please note: This plasmid contains a 129bp deletion in the exorh promoter. This deletion is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCK083 desmaMCS:minpromEGFP,exorh:mCherry was a gift from Christian Mosimann (Addgene plasmid # 200016 ; http://n2t.net/addgene:200016 ; RRID:Addgene_200016)

  • For your References section:

    Next-generation plasmids for transgenesis in zebrafish and beyond. Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, Lovely CB, Kwan KM, Burger A, Mosimann C. Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531. 10.1242/dev.201531 PubMed 36975217
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