Skip to main content
Addgene

Vector 1174D
(Plasmid #200012)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200012 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PiggyBac
  • Vector type
    Insect Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Engineered dobulesex splicing module
  • Species
    Synthetic; Aedes aegypti
  • Insert Size (bp)
    4515
  • Mutation
    Engineered dobulesex splicing module
  • GenBank ID
    NC_035107.1 AAEL009114
  • Promoter Hr5IE1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atcgtgaacaaccaagtgacttaattaaCAAAATGAACGACGAACTTGTCAAACGATCTC
  • 3′ sequencing primer GTTATCCTCCTCGCCCTTGCTCACCATTCCGGCGAGCTGGACTGTGACCGGGTAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Vector 1174D was a gift from Omar Akbari (Addgene plasmid # 200012 ; http://n2t.net/addgene:200012 ; RRID:Addgene_200012)

  • For your References section:

    Efficient Sex Separation by Exploiting Differential Alternative Splicing of a Dominant Marker in Aedes aegypti. Weng SC, Antoshechkin I, Marois E, Akbari OS. bioRxiv. 2023 Jun 16:2023.06.16.545348. doi: 10.1101/2023.06.16.545348. Preprint. 10.1101/2023.06.16.545348 PubMed 37398094
Related items:
Commonly requested with: