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Addgene

T7-Elongin C-pcDNA3
(Plasmid #19998)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19998 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5375
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha or XL1 @ 37˚C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Elongin C
  • Alt name
    TCEB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    415
  • GenBank ID
    NM_005648
  • Entrez Gene
    ELOC (a.k.a. SIII, TCEB1)
  • Tag / Fusion Protein
    • T7-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-Elongin C-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 19998 ; http://n2t.net/addgene:19998 ; RRID:Addgene_19998)

  • For your References section:

    Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor suppressor protein requires binding to complexes containing elongins B/C and Cul2. Lonergan KM, Iliopoulos O, Ohh M, Kamura T, Conaway RC, Conaway JW, Kaelin WG. Mol Cell Biol. 1998 Feb . 18(2):732-41. PubMed 9447969
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