pcDNA3-FLAG-Rheb-N153T
(Plasmid #19997)

• Depositing Lab: Fuyuhiko Tamanoi
• Publication: Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3514-9.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ras homolog enriched in brain 1 - N153T
• Alt name: Rheb-N153T
• Alt name: RHEB2; MGC111559; RHEB
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Mutation: Changed Asparagine 153 to Threonine
• Entrez Gene: RHEB (a.k.a. RHEB2)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry